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Celo.Cardiomyocytes

Celo.Cardiomyocytes are a highly pure and electrophysiologically active population of iPSC-derived cardiomyocytes, a product suitable for all types of applications.

2a2v DAPI_20ms_Celo.Cardiomyocytes

It's all about
Quality Cardiomyocytes 

Celo.Cardiomyocytes are suitable for all types of applications, including your next experiment. Our cells are derived from induced Pluripotent Stem Cells (iPSCs) under serum free conditions using proprietary protocols which optimize the user experience by prioritizing purity, reproducibility, and electrophysiology. With its serum free media kit, Celo.Cardiomyocytes would show the best possible result under 2D, 3D, and organoid cultures. We strive to provide fully functional human cardiomyocytes. 

Quality Cells

- ISO 9001-certified Quality Control

-Validated extensively in peer-reviewed journals and application notes.

-Electrophysiology-focused product development

Quality Research

- Consistent Results (low variability)

-High Sensitivity to electrophysiological changes from drug treatment.

-Serum-free media prevents unwanted interactions & increases biological relevance

Price
Celo.Cardiomyocytes 5M vial

Celo.Cardiomyocytes

One vial of

Celo.Cardiomyocytes 

1100

$

  • Celo.Cardiomyocytes (5x10^6 cells)

*We recommend using Celo.Cardiomyocytes with our specifically validated media for the best possible results.

Advanced medium

Media Kit

Extra bottle for your plated Celo.Cardiomyocytes

200

$

  • Advanced Media

- Basal medium (200mL)

- Advanced Supplement

  • Plating Media 

- Plating medium (45mL)

- Plating Supplement

$170 for Advanced Medium & Supplement only

Celo.Cardiomyocytes full kit

Complete Culture Kit

For your next iPSC-derived Cardiomyocytes research

1300

$

  • Celo.Cardiomyocytes (5x10^6 cells)

  • Advanced Media

- Basal medium (200mL)

- Advanced Supplement

  • Plating Media 

- Plating medium (45mL)

- Plating Supplement

* We recommend using Celo.Cardiomyocytes with our specifically validated media for the best possible results.

Data Figures

Validated Quality

Celo.Cardiomyocytes have been validated on multiple platforms (Curi Bio Mantaray, Axion Maestro, Nanion Cardioexcyte96, ACEA CardioECR, etc). The quality control process is central in ensuring both accurate and consistent results for our users.

Celo.Cardiomyocytes morphology

12-well culture plates
Images of seeded Celo.Cardiomyocytes on a 12-well culture plate throughout day 1 to 14 post-thawing (magnification 100x) at a plating density of 150,000 cells/㎠.

Celo.Cardiomyocytes recover rapidly upon thawing and beat spontaneously from day 2. The beating stabilizes over time (45~60 bpm on DIV7). Users can benefit from a single synchronous monolater formed by these cardiomyocytes which enhances both the reproducibility and accuracy of assays.​

 

48-well culture plate
Axion Maestro 48 well MEA plate DIV1

DIV 1

Axion Maestro 48 well MEA plate DIV3

DIV 3

Axion Maestro 48 well MEA plate DIV5

DIV 5

Axion Maestro 48 well MEA plate DIV7
Axion Maestro 48 well MEA plate DIV11
Axion Maestro 48 well MEA plate DIV14

DIV 7

DIV 11

DIV 14

Images of seeded Celo,Cardiomyocytes on a 48well Axion Citoview MEA plate throughout day 1 to 14 post-thawing (magnification 100x) at a plating density suggested on the Celo.Cardiomyocytes MEA User Guide.

Celo.Cardiomyocytes express
Ventricular cardiomyocyte-specific markers

Fluorescence microscopy images of Celo.Cardiomyocytes seeded on Matrigel-coated coverslip at a density of 30,000 cells. Cells were cultured and fixed on day 7.

Celo.Cardiomyocytes express typical ventricular cardiomyocyte markers and structural characteristics. In particular, the Celo.Cardiomyocytes demonstrate high expression of Connexin 43, a gap junction protein, which suggests high interconnectivity and synchronicity. 

Fluorescence staining

Celo.Cardiomyocytes demonstrates
Human cardiomyocyte-like electrophysiology

Patch Clamp (Nanion Patchliner)
img_pro-data-electrophy_01.jpg

Celo.Cardiomyocytes with Nanion Patchliner demonstrated a physiologically relevant and reproducible result when measuring the sodium currents (INa). (A) Dose-dependent block of sodium currents by TTX. (B) Late sodium currents were induced by ATX-II (enhancer) and Ranolazine (blocker). (C) Change in APD90 indicates calcium channel activation by BayK and inhibition by Nifedipine. These results demonstrate that Celo.Cardiomyocytes express ion channels similar to adult humans.

Dynamic Clamp (Nanion Patchliner Dynamite8)
Dynamic clamp (Nanion)

Celo.Cardiomyocytes with dynamic clamp experiments on the Nanion Patchliner with the Dynamite8 add-on. (A)  Adult-ventricular-like action potentials with the Celo.Cardiomyocytes under physiological temperature. (B) ,(C) Effects of calcium channel activation (BayK) and inhibition (Nifedipine) assessed in the presence of dynamic clamping.

CardioExcyte96
CardioExcyte96

Celo.Cardiomyocytes with Nanion CardioExcyte 96 show low inter-well variability with high reproducibility of impedance and EFP signals.  (A),(B) Compound testing experiments were performed using Nifedipine and Defetilide and dose-dependent results were observed according to the inotropic effect of each drug.

FLEXcyte96
FLEXcyte96

A soft substrate on Nanion’s FLEXcyte 96 induces maturation of the Celo.Cardiomyocytes. High responsiveness to Isoproterenol detected positive inotropic effects on day 7 of culture.

Axion Maestro (Spontaneous Baseline)
MEA baseline
Axion Maestro ( MEA drug response)

Celo.Cardiomyocytes on Axion Maestro show low variability, good synchronicity, and physiologically relevant compound reactivity toward Nifedipine, E4031, Dofetilide, and Mexiletine. Electrophysiology function of Celo.Cardiomyocytes are physiologically relevant to that of adult humans. Users are able to find the above information on the Certificate of Analysis for every batch they purchase. 

Download the Celo.Cardiomyocytes product brochure by clicking the image below.
For additional information, please contact us at  
celogics@celogics.com

Brochure
Product Brocure